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Image Search Results


Molecular docking diagrams of Z3 with Nrf2 (PDB:2FLU).

Journal: Frontiers in Pharmacology

Article Title: Synthesis, target analysis, and cerebroprotective effects of novel imide antioxidants via the Nrf2/HO-1 pathway in cerebral ischemia-reperfusion injury

doi: 10.3389/fphar.2025.1552717

Figure Lengend Snippet: Molecular docking diagrams of Z3 with Nrf2 (PDB:2FLU).

Article Snippet: The anti-p62 (Cat# sc-48402) and anti-Nrf2 (Cat# sc-365949) antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, United States), and the anti-mouse IgG (Cat# 7076S) antibody was from Cell Signaling Technology (Danvers, MA, United States).

Techniques:

Z3-mediated activation of the sestrin 2/p62/Nrf2/HO-1 signaling pathway in PC12 cells. (A) Immunofluorescence of cells pre-incubated with 10 μmol·L −1 Z3 or TBHQ for 6 h and stained with Nrf2 antibody (red) and DAPI (blue), showing the nuclear translocation of Nrf2. (B,C) Western blotting of sestrin 2, p62, and HO-1 in cells treated with Z3 for 24 h (D) MTT viability assays of cells pretreated with ZnPP for 1 h prior to the addition of Z3 and stimulation with H 2 O 2 . Data expressed as means ± SD (n = 3); #### P < 0.0001; ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Synthesis, target analysis, and cerebroprotective effects of novel imide antioxidants via the Nrf2/HO-1 pathway in cerebral ischemia-reperfusion injury

doi: 10.3389/fphar.2025.1552717

Figure Lengend Snippet: Z3-mediated activation of the sestrin 2/p62/Nrf2/HO-1 signaling pathway in PC12 cells. (A) Immunofluorescence of cells pre-incubated with 10 μmol·L −1 Z3 or TBHQ for 6 h and stained with Nrf2 antibody (red) and DAPI (blue), showing the nuclear translocation of Nrf2. (B,C) Western blotting of sestrin 2, p62, and HO-1 in cells treated with Z3 for 24 h (D) MTT viability assays of cells pretreated with ZnPP for 1 h prior to the addition of Z3 and stimulation with H 2 O 2 . Data expressed as means ± SD (n = 3); #### P < 0.0001; ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The anti-p62 (Cat# sc-48402) and anti-Nrf2 (Cat# sc-365949) antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, United States), and the anti-mouse IgG (Cat# 7076S) antibody was from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Activation Assay, Immunofluorescence, Incubation, Staining, Translocation Assay, Western Blot

Immunohistochemical detection of Nrf2 expression (x 400). ( A , B ) Control and vilda alone groups showed minimal immunostaining, respectively. ( C ) Ovaries from irradiated rats showed extensive brown immunostaining (increased cytoplasmic Nrf2). ( D ) Vilda co-administration resulted in a marked reduction in cytoplasmic Nrf2. ( E ) Quantitative image analysis for Nrf2 immunostaining was expressed as optical density (O.D.). ( F ) Scoring of the immunostaining considering both the staining intensity and the percentage of positive cells

Journal: BMC Pharmacology & Toxicology

Article Title: Can vildagliptin protect against radiation-induced premature ovarian failure? Insights into the AMPK and AKT signaling pathways

doi: 10.1186/s40360-025-00903-5

Figure Lengend Snippet: Immunohistochemical detection of Nrf2 expression (x 400). ( A , B ) Control and vilda alone groups showed minimal immunostaining, respectively. ( C ) Ovaries from irradiated rats showed extensive brown immunostaining (increased cytoplasmic Nrf2). ( D ) Vilda co-administration resulted in a marked reduction in cytoplasmic Nrf2. ( E ) Quantitative image analysis for Nrf2 immunostaining was expressed as optical density (O.D.). ( F ) Scoring of the immunostaining considering both the staining intensity and the percentage of positive cells

Article Snippet: The slides were then blocked with 5% bovine serum albumin in tris-buffered saline for 2 h. The sections were then immunostained with Nrf2 primary antibody: rabbit polyclonal anti-rat Nrf2 antibody (ABclonal, Cat No. A0674) at a concentration of 1 μg/ml containing 5% bovine serum albumin in tris-buffered saline and incubated overnight at 4 °C.

Techniques: Immunohistochemical staining, Expressing, Control, Immunostaining, Irradiation, Staining